Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5744 | 5' | -49.4 | NC_001806.1 | + | 70212 | 0.67 | 0.99609 |
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Target: 5'- uGCUCCGGGaGCUGUA-CGuGAU-CU-CCa -3' miRNA: 3'- -UGAGGUUC-CGACAUaGC-CUAaGAuGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 103488 | 0.68 | 0.992806 |
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Target: 5'- aACcCgGGGGCUGuUGUCGGGUggCUcccGCCg -3' miRNA: 3'- -UGaGgUUCCGAC-AUAGCCUAa-GA---UGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 38503 | 0.68 | 0.991711 |
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Target: 5'- gGCUgCCGucgGGGCUgggGUGUCGGAUaugGCCu -3' miRNA: 3'- -UGA-GGU---UCCGA---CAUAGCCUAagaUGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 107042 | 0.69 | 0.979935 |
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Target: 5'- cGCggggCCGcGGGCUGUGccgCGGAUUCUGu- -3' miRNA: 3'- -UGa---GGU-UCCGACAUa--GCCUAAGAUgg -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 12765 | 0.7 | 0.972172 |
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Target: 5'- --aCCAGGGCUGcca-GGAgaCUGCCg -3' miRNA: 3'- ugaGGUUCCGACauagCCUaaGAUGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 123073 | 0.7 | 0.972172 |
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Target: 5'- cGCUCCAGGuacuGCUGgacGUCGGAcgccagCUGCUg -3' miRNA: 3'- -UGAGGUUC----CGACa--UAGCCUaa----GAUGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 130076 | 0.71 | 0.958739 |
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Target: 5'- gGCUCCGGGGCgcgcUCGGAgcccgUCUuuacGCCc -3' miRNA: 3'- -UGAGGUUCCGacauAGCCUa----AGA----UGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 19420 | 0.74 | 0.840061 |
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Target: 5'- -aUCCGAuGCUGUcUCGGAUUCgACCu -3' miRNA: 3'- ugAGGUUcCGACAuAGCCUAAGaUGG- -5' |
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| 5744 | 5' | -49.4 | NC_001806.1 | + | 19448 | 1.13 | 0.007214 |
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Target: 5'- gACUCCAAGGCUGUAUCGGAUUCUACCu -3' miRNA: 3'- -UGAGGUUCCGACAUAGCCUAAGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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