miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
575 3' -48 AC_000015.1 + 31898 0.67 0.900842
Target:  5'- uGUGCGUGGGGAGagauugguguauUCUguuaaaugggaaGGGGGAgGCAa -3'
miRNA:   3'- -CACGUACCUUUU------------AGAa-----------CCUCCUgUGU- -5'
575 3' -48 AC_000015.1 + 16763 0.67 0.880915
Target:  5'- -gGCAUGGGcauccauGGAUCcguuugcacuucagUGGAGGugGCAg -3'
miRNA:   3'- caCGUACCU-------UUUAGa-------------ACCUCCugUGU- -5'
575 3' -48 AC_000015.1 + 4413 0.71 0.699364
Target:  5'- -aGCGUGGAAAAaUUUGGAG-ACACc -3'
miRNA:   3'- caCGUACCUUUUaGAACCUCcUGUGu -5'
575 3' -48 AC_000015.1 + 4499 1.1 0.002338
Target:  5'- aGUGCAUGGAAAAUCUUGGAGGACACAa -3'
miRNA:   3'- -CACGUACCUUUUAGAACCUCCUGUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.