Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5759 | 3' | -47.5 | NC_001806.1 | + | 145640 | 0.67 | 0.999209 |
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Target: 5'- -gGAGUGGCgGGug-CCGGggGACCGg -3' miRNA: 3'- caCUUAUUGgUCugaGGCCaaUUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 3356 | 0.67 | 0.999209 |
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Target: 5'- cUGGcgGGCCGGGCcCCGGccAGCCc -3' miRNA: 3'- cACUuaUUGGUCUGaGGCCaaUUGGu -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 34396 | 0.68 | 0.998241 |
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Target: 5'- gGUGGgcggcgGUGGCCGGGCcgggCCGGgccgGGCCGg -3' miRNA: 3'- -CACU------UAUUGGUCUGa---GGCCaa--UUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 131978 | 0.69 | 0.996423 |
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Target: 5'- cUGGAgcGCCAGACcgggCCGG-UGGCCc -3' miRNA: 3'- cACUUauUGGUCUGa---GGCCaAUUGGu -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 31727 | 0.7 | 0.992226 |
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Target: 5'- aGUGGGggugccauguUGGgCAGGCUCUGGUguUAACCAc -3' miRNA: 3'- -CACUU----------AUUgGUCUGAGGCCA--AUUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 14555 | 0.7 | 0.991041 |
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Target: 5'- -cGGAUAGCCAGGCcuggcucagacgUCC-GUUGGCCGu -3' miRNA: 3'- caCUUAUUGGUCUG------------AGGcCAAUUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 54543 | 0.71 | 0.984819 |
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Target: 5'- -gGAAUcccGGCCGGAUaUCCGGUcAACCGa -3' miRNA: 3'- caCUUA---UUGGUCUG-AGGCCAaUUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 57032 | 0.74 | 0.914186 |
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Target: 5'- cUGggUGGCCGGGC-CCGGg--GCCGg -3' miRNA: 3'- cACuuAUUGGUCUGaGGCCaauUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 88189 | 0.77 | 0.83885 |
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Target: 5'- cUGGAUGACCAGGucCUCCGGgugcccGGCCAc -3' miRNA: 3'- cACUUAUUGGUCU--GAGGCCaa----UUGGU- -5' |
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| 5759 | 3' | -47.5 | NC_001806.1 | + | 10619 | 1.1 | 0.016704 |
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Target: 5'- cGUGAAUAACCAGACUCCGGUUAACCAc -3' miRNA: 3'- -CACUUAUUGGUCUGAGGCCAAUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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