Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5759 | 5' | -52.5 | NC_001806.1 | + | 50486 | 0.66 | 0.985503 |
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Target: 5'- cCCUGCCcCugGgAAGccCCGcggUCGUGGg -3' miRNA: 3'- aGGACGGuGugC-UUCa-GGCa--AGCAUC- -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 81269 | 0.66 | 0.983684 |
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Target: 5'- cCCggGCCuCACGggGUcCCGggCGUc- -3' miRNA: 3'- aGGa-CGGuGUGCuuCA-GGCaaGCAuc -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 3205 | 0.66 | 0.979532 |
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Target: 5'- gCCgGCCACGCGcAGGUCCccgCGcAGg -3' miRNA: 3'- aGGaCGGUGUGC-UUCAGGcaaGCaUC- -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 86674 | 0.67 | 0.968029 |
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Target: 5'- gCCgugagGCCGCggcacagcacaaagAUGGAGUCCGUgucccCGUAGa -3' miRNA: 3'- aGGa----CGGUG--------------UGCUUCAGGCAa----GCAUC- -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 92682 | 0.68 | 0.946579 |
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Target: 5'- cUCCUGUCGCGCGAGGacaucgagaccaUCgCGUUCa--- -3' miRNA: 3'- -AGGACGGUGUGCUUC------------AG-GCAAGcauc -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 54483 | 0.68 | 0.937225 |
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Target: 5'- aCCUGCCGCucggUGggGUCCcUggGUGGg -3' miRNA: 3'- aGGACGGUGu---GCuuCAGGcAagCAUC- -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 139848 | 0.69 | 0.926885 |
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Target: 5'- gCCUGCCGCGCGGAGg-CGUUg---- -3' miRNA: 3'- aGGACGGUGUGCUUCagGCAAgcauc -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 137238 | 0.69 | 0.909519 |
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Target: 5'- gCCUGCCccuCAgGGAGuuUCCGUUCGcGGu -3' miRNA: 3'- aGGACGGu--GUgCUUC--AGGCAAGCaUC- -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 36585 | 0.7 | 0.88296 |
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Target: 5'- cUCCgcacgGCCACgcaaGCGGAGUCCGUUUu--- -3' miRNA: 3'- -AGGa----CGGUG----UGCUUCAGGCAAGcauc -5' |
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| 5759 | 5' | -52.5 | NC_001806.1 | + | 10656 | 1.1 | 0.005741 |
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Target: 5'- cUCCUGCCACACGAAGUCCGUUCGUAGa -3' miRNA: 3'- -AGGACGGUGUGCUUCAGGCAAGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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