Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5760 | 3' | -50.9 | NC_001806.1 | + | 6572 | 0.66 | 0.991673 |
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Target: 5'- --aGGAGggggcucacccGCGUUCGUGCcuucccgcaggagGAACGUCc -3' miRNA: 3'- cuaCCUC-----------UGCAAGCACGa------------CUUGCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 11306 | 0.66 | 0.990962 |
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Target: 5'- --gGGAGGCGUUgG-GgUGggUGUCg -3' miRNA: 3'- cuaCCUCUGCAAgCaCgACuuGCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 148907 | 0.66 | 0.990962 |
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Target: 5'- --cGGAGGCGgagUCGUcGUcaUGGugGUCg -3' miRNA: 3'- cuaCCUCUGCa--AGCA-CG--ACUugCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 66228 | 0.66 | 0.990962 |
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Target: 5'- -uUGGGGGCGgagcgUUGUGUgggcgGGGCGUUUg -3' miRNA: 3'- cuACCUCUGCa----AGCACGa----CUUGCAGA- -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 148540 | 0.66 | 0.989671 |
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Target: 5'- -cUGGGGGuCGUUCacgaucgggaugGUGCUGAACGa-- -3' miRNA: 3'- cuACCUCU-GCAAG------------CACGACUUGCaga -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 15971 | 0.68 | 0.973542 |
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Target: 5'- --cGGGGGCGUUgGUGUcGAugGUUc -3' miRNA: 3'- cuaCCUCUGCAAgCACGaCUugCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 94300 | 0.7 | 0.934369 |
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Target: 5'- -cUGGAGACGUUCGcgaccGCggGAgACGUCg -3' miRNA: 3'- cuACCUCUGCAAGCa----CGa-CU-UGCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 135111 | 0.75 | 0.709962 |
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Target: 5'- --gGGGGGCGggggcagcgcugCGUGCUGGACGUCc -3' miRNA: 3'- cuaCCUCUGCaa----------GCACGACUUGCAGa -5' |
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| 5760 | 3' | -50.9 | NC_001806.1 | + | 10271 | 1.07 | 0.011415 |
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Target: 5'- gGAUGGAGACGUUCGUGCUGAACGUCUc -3' miRNA: 3'- -CUACCUCUGCAAGCACGACUUGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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