Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5764 | 5' | -51.8 | NC_001806.1 | + | 9203 | 0.66 | 0.984921 |
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Target: 5'- -gGUgUGGCGaggGCGCgGUCGACGUGg- -3' miRNA: 3'- gaCAgACUGUg--UGCG-UAGCUGCACaa -5' |
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| 5764 | 5' | -51.8 | NC_001806.1 | + | 22436 | 0.66 | 0.983015 |
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Target: 5'- gCUGgggcCUGGCGCACGCggCGGCcgccGUGg- -3' miRNA: 3'- -GACa---GACUGUGUGCGuaGCUG----CACaa -5' |
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| 5764 | 5' | -51.8 | NC_001806.1 | + | 116214 | 0.67 | 0.976205 |
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Target: 5'- ---aCUGACGCugGC--CGACGUGg- -3' miRNA: 3'- gacaGACUGUGugCGuaGCUGCACaa -5' |
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| 5764 | 5' | -51.8 | NC_001806.1 | + | 75036 | 0.67 | 0.976205 |
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Target: 5'- uCUGUCUGGCuuGCAUcccaGCuUCGugGUGg- -3' miRNA: 3'- -GACAGACUG--UGUG----CGuAGCugCACaa -5' |
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| 5764 | 5' | -51.8 | NC_001806.1 | + | 42128 | 0.69 | 0.939387 |
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Target: 5'- -gGUCgGACACGCGCAggcaacugCGAuCGUGg- -3' miRNA: 3'- gaCAGaCUGUGUGCGUa-------GCU-GCACaa -5' |
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| 5764 | 5' | -51.8 | NC_001806.1 | + | 7984 | 1 | 0.023973 |
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Target: 5'- -cGUCUGACACACGCAUCGACGUGUUc -3' miRNA: 3'- gaCAGACUGUGUGCGUAGCUGCACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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