Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5768 | 3' | -56 | NC_001806.1 | + | 120248 | 0.66 | 0.911303 |
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Target: 5'- -----uGCGCGCCgUCGAGGGgacCGCCCc -3' miRNA: 3'- cacaagUGCGUGG-AGCUCCU---GUGGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 76854 | 0.66 | 0.911303 |
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Target: 5'- -aGggCGCGCGCCUgcAGGcgcucuucgACACCCGu -3' miRNA: 3'- caCaaGUGCGUGGAgcUCC---------UGUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 100718 | 0.67 | 0.871488 |
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Target: 5'- cGUGUgggUCA-GCGCCUCcacgccGGGCGCCCa -3' miRNA: 3'- -CACA---AGUgCGUGGAGcu----CCUGUGGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 21312 | 0.67 | 0.864082 |
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Target: 5'- -cGggCAcCGaCGCCggCGAGGACGCCgGg -3' miRNA: 3'- caCaaGU-GC-GUGGa-GCUCCUGUGGgC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 128698 | 0.67 | 0.856469 |
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Target: 5'- ----cCGCGCACCUCGGccguGGAguuCACCCc -3' miRNA: 3'- cacaaGUGCGUGGAGCU----CCU---GUGGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 69976 | 0.67 | 0.856469 |
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Target: 5'- -cGUUCugGC-CCugguggUCGGGGACGCgCUGa -3' miRNA: 3'- caCAAGugCGuGG------AGCUCCUGUG-GGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 4405 | 0.68 | 0.848655 |
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Target: 5'- uUGggCGCG-GCCUCGgagaggggGGGugGCCCGg -3' miRNA: 3'- cACaaGUGCgUGGAGC--------UCCugUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 7099 | 1.08 | 0.003817 |
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Target: 5'- gGUGUUCACGCACCUCGAGGACACCCGc -3' miRNA: 3'- -CACAAGUGCGUGGAGCUCCUGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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