Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5768 | 3' | -56 | NC_001806.1 | + | 371 | 0.7 | 0.741901 |
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Target: 5'- -cGcgCGCGCGCCcgCGGGGG-GCCCGg -3' miRNA: 3'- caCaaGUGCGUGGa-GCUCCUgUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 30402 | 0.7 | 0.741901 |
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Target: 5'- -gGUggCGCGCGgCUCGGGGGgGCCgGg -3' miRNA: 3'- caCAa-GUGCGUgGAGCUCCUgUGGgC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 152081 | 0.7 | 0.741901 |
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Target: 5'- -cGcgCGCGCGCCcgCGGGGG-GCCCGg -3' miRNA: 3'- caCaaGUGCGUGGa-GCUCCUgUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 23507 | 0.7 | 0.712478 |
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Target: 5'- cGUG-UCGCGCcgGCCcgcCGAGGGC-CCCGa -3' miRNA: 3'- -CACaAGUGCG--UGGa--GCUCCUGuGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 54711 | 0.76 | 0.401848 |
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Target: 5'- ---gUCACGC-CCUCGGGGACGCagCCGg -3' miRNA: 3'- cacaAGUGCGuGGAGCUCCUGUG--GGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 24454 | 0.78 | 0.322686 |
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Target: 5'- cGUGcgCACGCGCUUCGGcccGGACACgCCGg -3' miRNA: 3'- -CACaaGUGCGUGGAGCU---CCUGUG-GGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 71002 | 0.79 | 0.281065 |
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Target: 5'- aUGgcgCAUGCGCC-CGAGGACGCCCc -3' miRNA: 3'- cACaa-GUGCGUGGaGCUCCUGUGGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 7099 | 1.08 | 0.003817 |
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Target: 5'- gGUGUUCACGCACCUCGAGGACACCCGc -3' miRNA: 3'- -CACAAGUGCGUGGAGCUCCUGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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