Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5780 | 5' | -50.7 | NC_001806.1 | + | 119139 | 0.66 | 0.991136 |
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Target: 5'- cGCGCAuCAUGCgcGUCUGUCGGcAUUUg -3' miRNA: 3'- -UGCGU-GUACGaaCGGACAGUU-UGAGa -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 50547 | 0.66 | 0.988396 |
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Target: 5'- aGCGCGCcgGCUgcucGCCaacagCGGGCUCa -3' miRNA: 3'- -UGCGUGuaCGAa---CGGaca--GUUUGAGa -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 109835 | 0.67 | 0.980992 |
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Target: 5'- gACGCACGuccUGCagGCCgcguUGUCgGAGCUCg -3' miRNA: 3'- -UGCGUGU---ACGaaCGG----ACAG-UUUGAGa -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 139188 | 0.68 | 0.973455 |
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Target: 5'- uACGCACggGUGUUUGCCUuugCGGACgagCUg -3' miRNA: 3'- -UGCGUG--UACGAACGGAca-GUUUGa--GA- -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 88708 | 0.68 | 0.960325 |
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Target: 5'- gGCGCgGCAuUGUaggUGCCUGUCGAcCUCc -3' miRNA: 3'- -UGCG-UGU-ACGa--ACGGACAGUUuGAGa -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 151646 | 0.81 | 0.380662 |
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Target: 5'- uGCGUGCAgaggcgaguagUGCUUGCCUGUCuAACUCg -3' miRNA: 3'- -UGCGUGU-----------ACGAACGGACAGuUUGAGa -5' |
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| 5780 | 5' | -50.7 | NC_001806.1 | + | 26325 | 1.09 | 0.008077 |
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Target: 5'- cACGCACAUGCUUGCCUGUCAAACUCUa -3' miRNA: 3'- -UGCGUGUACGAACGGACAGUUUGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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