Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5784 | 5' | -51.2 | NC_001806.1 | + | 147688 | 0.66 | 0.990422 |
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Target: 5'- gGCCggggcgcGGggGCGGGCCCCggaggcggcgCUCg -3' miRNA: 3'- gUGGaaa----CCaaCGUCUGGGGaaa-------GAG- -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 50961 | 0.66 | 0.986219 |
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Target: 5'- -uCCg--GGacgGCAGACCCCgccuuucUUCUCa -3' miRNA: 3'- guGGaaaCCaa-CGUCUGGGGa------AAGAG- -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 79838 | 0.66 | 0.986219 |
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Target: 5'- cCAgCUUUGGUgucUGguGuGCCCCUcccgCUCg -3' miRNA: 3'- -GUgGAAACCA---ACguC-UGGGGAaa--GAG- -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 45690 | 0.66 | 0.984417 |
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Target: 5'- -cCCagUGGguuuUGCGGACCCuCUcUUCUCu -3' miRNA: 3'- guGGaaACCa---ACGUCUGGG-GA-AAGAG- -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 103776 | 0.67 | 0.969644 |
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Target: 5'- gACCUUUucGGUcugcUGCAGACCCCc----- -3' miRNA: 3'- gUGGAAA--CCA----ACGUCUGGGGaaagag -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 63318 | 0.7 | 0.89549 |
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Target: 5'- aCGCUUcccaGGUUGCAGACCCCa----- -3' miRNA: 3'- -GUGGAaa--CCAACGUCUGGGGaaagag -5' |
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| 5784 | 5' | -51.2 | NC_001806.1 | + | 28790 | 1.09 | 0.007072 |
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Target: 5'- cCACCUUUGGUUGCAGACCCCUUUCUCc -3' miRNA: 3'- -GUGGAAACCAACGUCUGGGGAAAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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