miRNA: 3'- -CGGCGGUGGUa--GGag-UUCGuCCG-GUa -5'
miRNA: 3'- -CGGCGGuGGuaGGAGUuc------GUCCGGUA- -5'
miRNA: 3'- -CGGCGGUGGUaGGAGUUcgUCCGGUa -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5803 | 5' | -58.7 | NC_001806.1 | + | 144207 | 0.66 | 0.762065 |
|
Target: 5'- aGCCGCCGCCGacaCCgcaGAGCcGGCgCGc -3' miRNA: 3'- -CGGCGGUGGUa--GGag-UUCGuCCG-GUa -5' |
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| 5803 | 5' | -58.7 | NC_001806.1 | + | 148347 | 0.67 | 0.713359 |
|
Target: 5'- uGCCGCCcCCcgCCUCAccgucgucCAGGUCGUc -3' miRNA: 3'- -CGGCGGuGGuaGGAGUuc------GUCCGGUA- -5' |
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| 5803 | 5' | -58.7 | NC_001806.1 | + | 151714 | 0.69 | 0.571235 |
|
Target: 5'- cGCCGCCACCG-CUUUAAa-GGGCCGc -3' miRNA: 3'- -CGGCGGUGGUaGGAGUUcgUCCGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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