miRNA: 3'- -CugCu--CAAGCAGGa-CGGGGUgACCAU- -5'
miRNA: 3'- cUGCUca--AGCaGGACGGGGuGACCau -5'
miRNA: 3'- cUGCUCAAGCaggaCGGGGUGACCau -5'
miRNA: 3'- -CUGCUCAagCAGGa------CGGGGUG-ACCau -5'
miRNA: 3'- cuGCU-CAAGCAGGACGGGGugACCau -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5804 | 3' | -57 | NC_001806.1 | + | 108344 | 0.67 | 0.827768 |
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Target: 5'- uGugGuuuGUUUGUUCagGCCCCGgUGGUGc -3' miRNA: 3'- -CugCu--CAAGCAGGa-CGGGGUgACCAU- -5' |
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| 5804 | 3' | -57 | NC_001806.1 | + | 116336 | 0.67 | 0.79266 |
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Target: 5'- cGCGAaucgUCG-CCUGCCCC-CUGGa- -3' miRNA: 3'- cUGCUca--AGCaGGACGGGGuGACCau -5' |
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| 5804 | 3' | -57 | NC_001806.1 | + | 111775 | 0.67 | 0.783499 |
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Target: 5'- cGCGAGUUCGac--GCCCUGCUGGg- -3' miRNA: 3'- cUGCUCAAGCaggaCGGGGUGACCau -5' |
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| 5804 | 3' | -57 | NC_001806.1 | + | 5676 | 0.68 | 0.774201 |
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Target: 5'- gGGCGGGUggGUCCgcgccccGCCCCGCaUGGc- -3' miRNA: 3'- -CUGCUCAagCAGGa------CGGGGUG-ACCau -5' |
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| 5804 | 3' | -57 | NC_001806.1 | + | 112818 | 0.71 | 0.604117 |
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Target: 5'- -cUGAcGUUCGUCCUGCCCC--UGGg- -3' miRNA: 3'- cuGCU-CAAGCAGGACGGGGugACCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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