Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 582 | 5' | -56.2 | AC_000015.1 | + | 1037 | 0.66 | 0.516465 |
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Target: 5'- gCCUUCaCUCcCUCACCCGcugcAGUCU-GCa -3' miRNA: 3'- gGGAAG-GAGaGGGUGGGU----UCAGGuCG- -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 26744 | 0.67 | 0.474298 |
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Target: 5'- -aCUUCCUCgagacgCCCagGCCgAAGUCCAa- -3' miRNA: 3'- ggGAAGGAGa-----GGG--UGGgUUCAGGUcg -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 12012 | 0.67 | 0.464025 |
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Target: 5'- gCCUUCCcgcucuaaUCCCACUCAugagaaGGUCCuGGCc -3' miRNA: 3'- gGGAAGGag------AGGGUGGGU------UCAGG-UCG- -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 23680 | 0.68 | 0.447834 |
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Target: 5'- aCUCUUCCUCguguUCCaccggugucacauaGCCCGGGUCUuGCu -3' miRNA: 3'- -GGGAAGGAG----AGGg-------------UGGGUUCAGGuCG- -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 33226 | 0.69 | 0.395623 |
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Target: 5'- uCCCUcUCCUCUCCUGCUUGAaacacaaUCCAGUu -3' miRNA: 3'- -GGGA-AGGAGAGGGUGGGUUc------AGGUCG- -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 10834 | 0.69 | 0.377307 |
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Target: 5'- gCCagUCCUUcgCCCucuUCCAAGUCCAGa -3' miRNA: 3'- gGGa-AGGAGa-GGGu--GGGUUCAGGUCg -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 21333 | 0.69 | 0.350923 |
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Target: 5'- aCCagUgCUCUCCC-CCCGucucacGUCCGGCc -3' miRNA: 3'- gGGa-AgGAGAGGGuGGGUu-----CAGGUCG- -5' |
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| 582 | 5' | -56.2 | AC_000015.1 | + | 13577 | 1.14 | 0.000202 |
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Target: 5'- cCCCUUCCUCUCCCACCCAAGUCCAGCa -3' miRNA: 3'- -GGGAAGGAGAGGGUGGGUUCAGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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