Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5830 | 3' | -61.8 | NC_001806.1 | + | 119060 | 0.7 | 0.41541 |
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Target: 5'- -gGGGCGaGCUgugCCCGCGUGAU--UCUg -3' miRNA: 3'- gaCCCGC-CGGa--GGGCGCACUGugAGA- -5' |
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| 5830 | 3' | -61.8 | NC_001806.1 | + | 26948 | 0.71 | 0.390657 |
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Target: 5'- uCUGGGCcucGGCCgCCCGCcUGGCGCg-- -3' miRNA: 3'- -GACCCG---CCGGaGGGCGcACUGUGaga -5' |
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| 5830 | 3' | -61.8 | NC_001806.1 | + | 147049 | 0.71 | 0.366112 |
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Target: 5'- -gGGGaCGGCCccggaagUCuCCGCGUGGCGCgUCUu -3' miRNA: 3'- gaCCC-GCCGG-------AG-GGCGCACUGUG-AGA- -5' |
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| 5830 | 3' | -61.8 | NC_001806.1 | + | 24637 | 0.72 | 0.344132 |
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Target: 5'- cCUGGGCGcGCCgcugcggCCCGUguacGUGGCGCUg- -3' miRNA: 3'- -GACCCGC-CGGa------GGGCG----CACUGUGAga -5' |
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| 5830 | 3' | -61.8 | NC_001806.1 | + | 86092 | 0.72 | 0.336775 |
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Target: 5'- -aGGGCGGCCagCCGCGcGACcguCUCc -3' miRNA: 3'- gaCCCGCCGGagGGCGCaCUGu--GAGa -5' |
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| 5830 | 3' | -61.8 | NC_001806.1 | + | 78687 | 0.72 | 0.329534 |
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Target: 5'- cCUGGGCGcGCCcgUCGuCGUGGCGCUCc -3' miRNA: 3'- -GACCCGC-CGGagGGC-GCACUGUGAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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