Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5841 | 3' | -57.5 | NC_001806.1 | + | 98022 | 0.7 | 0.653248 |
Target: 5'- -cUCGGCcacCGUGGGCCGGcgggugaGCGCGCGg -3' miRNA: 3'- caGGCCGa--GCAUCCGGCCa------UGUGUGU- -5' |
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5841 | 3' | -57.5 | NC_001806.1 | + | 113554 | 0.7 | 0.632974 |
Target: 5'- -cCCGGCUCGUGGuCCaGUGCAuCACc -3' miRNA: 3'- caGGCCGAGCAUCcGGcCAUGU-GUGu -5' |
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5841 | 3' | -57.5 | NC_001806.1 | + | 57138 | 0.7 | 0.629931 |
Target: 5'- aUCCGcGCg-GUGGGCCgcgcgaggggagggGGUGCGCGCAu -3' miRNA: 3'- cAGGC-CGagCAUCCGG--------------CCAUGUGUGU- -5' |
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5841 | 3' | -57.5 | NC_001806.1 | + | 46144 | 0.72 | 0.542539 |
Target: 5'- gGUCCGGag---GGGCCGG-ACGCACAc -3' miRNA: 3'- -CAGGCCgagcaUCCGGCCaUGUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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