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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
588 | 5' | -48.2 | AC_000015.1 | + | 2175 | 0.66 | 0.904584 |
Target: 5'- -cUCCUCCAcuGGAgggUCCAgGCcgGCUCu -3' miRNA: 3'- acAGGAGGU--UCUaaaAGGUaCG--UGAG- -5' |
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588 | 5' | -48.2 | AC_000015.1 | + | 1470 | 0.68 | 0.82557 |
Target: 5'- uUGUCUU---GGAcgUUUCCGUGUACUCa -3' miRNA: 3'- -ACAGGAgguUCUa-AAAGGUACGUGAG- -5' |
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588 | 5' | -48.2 | AC_000015.1 | + | 17488 | 0.68 | 0.82557 |
Target: 5'- aUGUCgCUCCAGGugc--CCAUGUACg- -3' miRNA: 3'- -ACAG-GAGGUUCuaaaaGGUACGUGag -5' |
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588 | 5' | -48.2 | AC_000015.1 | + | 4440 | 1.12 | 0.001661 |
Target: 5'- gUGUCCUCCAAGAUUUUCCAUGCACUCa -3' miRNA: 3'- -ACAGGAGGUUCUAAAAGGUACGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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