Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5885 | 3' | -56.7 | NC_001806.1 | + | 143118 | 0.66 | 0.857195 |
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Target: 5'- gGGGCGUGg-CUGCCGggaggggcCGCGgaUGGGCGGg -3' miRNA: 3'- -UUUGCACgaGACGGU--------GUGC--ACCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 110206 | 0.66 | 0.849303 |
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Target: 5'- --uCGUGCg-UGCCGCACGcgguggGGGcCGAa -3' miRNA: 3'- uuuGCACGagACGGUGUGCa-----CCC-GCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 26808 | 0.66 | 0.849303 |
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Target: 5'- -cGCGccUGCgcCUGCgACGCGcGGGCGGg -3' miRNA: 3'- uuUGC--ACGa-GACGgUGUGCaCCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 147665 | 0.67 | 0.832929 |
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Target: 5'- cGGGCGgccgGCUCcGCCccgggggccgggGCGCGgGGGCGGg -3' miRNA: 3'- -UUUGCa---CGAGaCGG------------UGUGCaCCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 92890 | 0.67 | 0.832929 |
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Target: 5'- cAGCGUGCaggCUGCgGcCGCGUgguccgcgcaGGGCGGg -3' miRNA: 3'- uUUGCACGa--GACGgU-GUGCA----------CCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 15606 | 0.68 | 0.760683 |
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Target: 5'- aAAGCa-GCUCUGCgGCGCuG-GGGCGAa -3' miRNA: 3'- -UUUGcaCGAGACGgUGUG-CaCCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 95280 | 0.69 | 0.691259 |
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Target: 5'- -cGCGUGCUg-GCgCGCAUGgGGGCGGg -3' miRNA: 3'- uuUGCACGAgaCG-GUGUGCaCCCGCU- -5' |
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| 5885 | 3' | -56.7 | NC_001806.1 | + | 36190 | 0.74 | 0.432858 |
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Target: 5'- uGACGcgucgaUGCUCUGCCGCGC--GGGCGGc -3' miRNA: 3'- uUUGC------ACGAGACGGUGUGcaCCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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