Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
589 | 3' | -49.7 | AC_000015.1 | + | 25131 | 0.66 | 0.826738 |
Target: 5'- aGGUAGCAGUgacuccauAGCGGCGGggggcacUCGCGGuAGg -3' miRNA: 3'- -UCGUCGUCG--------UCGUCGUU-------AGUGUUuUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 9328 | 0.66 | 0.817591 |
Target: 5'- cGCGGCGGCGGC-GCAugguuUCAguGAc- -3' miRNA: 3'- uCGUCGUCGUCGuCGUu----AGUguUUuc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 13212 | 0.66 | 0.807201 |
Target: 5'- cGCGGUAGCacccaugaccccGGaCAGCGAU-GCAGAAGa -3' miRNA: 3'- uCGUCGUCG------------UC-GUCGUUAgUGUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 15808 | 0.67 | 0.796587 |
Target: 5'- cGCAGgcaAGCAGCcGCuGUCGCAGcGGc -3' miRNA: 3'- uCGUCg--UCGUCGuCGuUAGUGUUuUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 12985 | 0.67 | 0.796587 |
Target: 5'- uAGCGGCAGCucuguGCaAGUAGUC-CAGcAGu -3' miRNA: 3'- -UCGUCGUCGu----CG-UCGUUAGuGUUuUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 16940 | 0.67 | 0.752179 |
Target: 5'- uGCAGguGUcuuGCGGCGAcgGCGGGAGg -3' miRNA: 3'- uCGUCguCGu--CGUCGUUagUGUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 4493 | 0.68 | 0.729015 |
Target: 5'- gGGCAGCGGC-GCGgGCAAaCACGu--- -3' miRNA: 3'- -UCGUCGUCGuCGU-CGUUaGUGUuuuc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 26135 | 0.68 | 0.705378 |
Target: 5'- -cCAGCAGCGGCAGUuagaaAAUaCACAAcAAGu -3' miRNA: 3'- ucGUCGUCGUCGUCG-----UUA-GUGUU-UUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 25220 | 0.68 | 0.69342 |
Target: 5'- uGCAGCGGCAGUgacacuccAGCAAgccgucuccgcUCACAu--- -3' miRNA: 3'- uCGUCGUCGUCG--------UCGUU-----------AGUGUuuuc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 32793 | 0.68 | 0.69342 |
Target: 5'- uGGCAaCAGCGGguGgucuuCAGUCAUAGAAGc -3' miRNA: 3'- -UCGUcGUCGUCguC-----GUUAGUGUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 1924 | 0.69 | 0.68139 |
Target: 5'- aAGCcaCAGCAGCAGCAGUUcuACcuGGGg -3' miRNA: 3'- -UCGucGUCGUCGUCGUUAG--UGuuUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 23247 | 0.69 | 0.63284 |
Target: 5'- gGGCAGaGGUGGaGGCGAUUGCGAAGGg -3' miRNA: 3'- -UCGUCgUCGUCgUCGUUAGUGUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 7960 | 0.7 | 0.596368 |
Target: 5'- cGCAGUAGUcGCAGCGuugCACGGGu- -3' miRNA: 3'- uCGUCGUCGuCGUCGUua-GUGUUUuc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 30571 | 0.7 | 0.584269 |
Target: 5'- cAGCAgGguGUAGguGCAcucgauggaAUCGCAGGAGc -3' miRNA: 3'- -UCGU-CguCGUCguCGU---------UAGUGUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 28760 | 0.72 | 0.501526 |
Target: 5'- cAGCGGUGGCAGUGGUGAUgGCAc--- -3' miRNA: 3'- -UCGUCGUCGUCGUCGUUAgUGUuuuc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 11678 | 0.73 | 0.445684 |
Target: 5'- gAGCugacuuGCAGUGGCAGCcuAGUCGCAGGGc -3' miRNA: 3'- -UCGu-----CGUCGUCGUCG--UUAGUGUUUUc -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 18000 | 0.73 | 0.413892 |
Target: 5'- -aCAGCAGUAGCAGCAGggGgAGGGGg -3' miRNA: 3'- ucGUCGUCGUCGUCGUUagUgUUUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 13739 | 0.74 | 0.358448 |
Target: 5'- uGCAGCAGCAGCAggcgacggcggugauGCAAUCcccACugGAGg -3' miRNA: 3'- uCGUCGUCGUCGU---------------CGUUAG---UGuuUUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 27258 | 0.77 | 0.234036 |
Target: 5'- aAGCAGCgaaAGCGGCGGCAguggucuagauGUCGCAGccGAGa -3' miRNA: 3'- -UCGUCG---UCGUCGUCGU-----------UAGUGUU--UUC- -5' |
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589 | 3' | -49.7 | AC_000015.1 | + | 10776 | 0.79 | 0.185025 |
Target: 5'- cGGCGGCAGUuGCAGUAGUUGCAGGGa -3' miRNA: 3'- -UCGUCGUCGuCGUCGUUAGUGUUUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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