Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5925 | 3' | -56.6 | NC_001806.1 | + | 23956 | 0.66 | 0.908247 |
Target: 5'- -gGggCCGCCGGaGUGGUccgcCGAGcgCGGc -3' miRNA: 3'- gaCaaGGCGGUC-CGCCA----GCUCuaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 74079 | 0.66 | 0.902102 |
Target: 5'- -aGgcCCGUCGGGCGaUCGAGGgcgCGGu -3' miRNA: 3'- gaCaaGGCGGUCCGCcAGCUCUa--GCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 73118 | 0.67 | 0.868028 |
Target: 5'- -cGggCCGCCggucgccgAGGCGGgCGAGGaCGAc -3' miRNA: 3'- gaCaaGGCGG--------UCCGCCaGCUCUaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 5654 | 0.67 | 0.860572 |
Target: 5'- ---aUCUGCCauGGGCGGggcgCGAGggCGGg -3' miRNA: 3'- gacaAGGCGG--UCCGCCa---GCUCuaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 136785 | 0.67 | 0.852137 |
Target: 5'- cCUGcagCUGCCGGGUGGUCGccucgcuGGAcCGGa -3' miRNA: 3'- -GACaa-GGCGGUCCGCCAGC-------UCUaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 145412 | 0.67 | 0.845061 |
Target: 5'- gCUGgaagaCCGCCAGGgGGUCGGccgguGUCGc -3' miRNA: 3'- -GACaa---GGCGGUCCgCCAGCUc----UAGCu -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 2717 | 0.68 | 0.828794 |
Target: 5'- ----gCCGcCCAGGgGGUCGGGGcccUCGGc -3' miRNA: 3'- gacaaGGC-GGUCCgCCAGCUCU---AGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 92190 | 0.68 | 0.828794 |
Target: 5'- gUGUU-CGCCGGGCagucGGUCGAGGggCGc -3' miRNA: 3'- gACAAgGCGGUCCG----CCAGCUCUa-GCu -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 67522 | 0.68 | 0.828794 |
Target: 5'- cCUGggCCGUCGcGGCGa--GAGAUCGGg -3' miRNA: 3'- -GACaaGGCGGU-CCGCcagCUCUAGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 151270 | 0.68 | 0.811827 |
Target: 5'- -----aCGCCGGGCGGUgGGGGcCGGg -3' miRNA: 3'- gacaagGCGGUCCGCCAgCUCUaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 69600 | 0.68 | 0.785202 |
Target: 5'- ---cUCUGCgAGGCGGUCGGccuGUCGGg -3' miRNA: 3'- gacaAGGCGgUCCGCCAGCUc--UAGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 21764 | 0.7 | 0.679032 |
Target: 5'- -cGggCCGUCGGGCGGgguccgUCGAGcgCGGc -3' miRNA: 3'- gaCaaGGCGGUCCGCC------AGCUCuaGCU- -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 4970 | 0.71 | 0.618438 |
Target: 5'- -cGUcCCGCCGGGCGucGUCGAG-UCGu -3' miRNA: 3'- gaCAaGGCGGUCCGC--CAGCUCuAGCu -5' |
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5925 | 3' | -56.6 | NC_001806.1 | + | 38279 | 0.72 | 0.598239 |
Target: 5'- -cGgaCCGCCGGGCGagcggcGUCGAGuAUCGGc -3' miRNA: 3'- gaCaaGGCGGUCCGC------CAGCUC-UAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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