Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5927 | 5' | -47 | NC_001806.1 | + | 94275 | 0.66 | 0.999819 |
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Target: 5'- cGAGgGCGAgCGGCUCCuggGCAGccUGGa -3' miRNA: 3'- -UUUgUGUUgGCCGAGGua-UGUUa-ACU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 5434 | 0.67 | 0.999397 |
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Target: 5'- ---gGCAGCCGcGCUCCGUguggACGAUcGGg -3' miRNA: 3'- uuugUGUUGGC-CGAGGUA----UGUUAaCU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 134633 | 0.68 | 0.998602 |
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Target: 5'- gGGGCACGGCCaGGCU--GUGCAcgUGGu -3' miRNA: 3'- -UUUGUGUUGG-CCGAggUAUGUuaACU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 51496 | 0.68 | 0.998301 |
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Target: 5'- cAACACAACaCGGUUCCcgAgAGuUUGAu -3' miRNA: 3'- uUUGUGUUG-GCCGAGGuaUgUU-AACU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 135640 | 0.68 | 0.997053 |
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Target: 5'- --uCGCggUCGcGCUCC-UGCAAUUGGg -3' miRNA: 3'- uuuGUGuuGGC-CGAGGuAUGUUAACU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 88865 | 0.68 | 0.997053 |
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Target: 5'- gGAACugGGCCGcGCgcaugCCGUACGcgUGc -3' miRNA: 3'- -UUUGugUUGGC-CGa----GGUAUGUuaACu -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 53178 | 0.69 | 0.994292 |
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Target: 5'- cGAAUGCAgcGCCGGCcuccCCGUACA-UUGAg -3' miRNA: 3'- -UUUGUGU--UGGCCGa---GGUAUGUuAACU- -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 54723 | 0.7 | 0.989742 |
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Target: 5'- gGGACGCAGCCGGC--CGUGCAGa--- -3' miRNA: 3'- -UUUGUGUUGGCCGagGUAUGUUaacu -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 136108 | 0.72 | 0.969578 |
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Target: 5'- -uGCGCAGCCGGCagaCGUGCGcgUGu -3' miRNA: 3'- uuUGUGUUGGCCGag-GUAUGUuaACu -5' |
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| 5927 | 5' | -47 | NC_001806.1 | + | 52097 | 0.94 | 0.137519 |
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Target: 5'- cGGCACGACCGGCUCCAUGCAGUg-- -3' miRNA: 3'- uUUGUGUUGGCCGAGGUAUGUUAacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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