Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5931 | 5' | -56.7 | NC_001806.1 | + | 48986 | 0.66 | 0.876377 |
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Target: 5'- uCCC-AUCgcGAGGGGGUCC-GGCGGc -3' miRNA: 3'- -GGGcUGGauCUCCUCCAGGaCUGCUu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 4409 | 0.66 | 0.869135 |
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Target: 5'- gCgCGGCCUcggAGAGGGGGgguggCCcgGGCGGGg -3' miRNA: 3'- -GgGCUGGA---UCUCCUCCa----GGa-CUGCUU- -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 141681 | 0.67 | 0.854028 |
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Target: 5'- gCCUGGCCggGGuGGugauauGGGUCCUGGcCGAu -3' miRNA: 3'- -GGGCUGGa-UCuCC------UCCAGGACU-GCUu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 43303 | 0.68 | 0.786253 |
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Target: 5'- gCCgCGGCUgc-AGGAGGcCCUGGCGGu -3' miRNA: 3'- -GG-GCUGGaucUCCUCCaGGACUGCUu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 120183 | 0.68 | 0.76778 |
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Target: 5'- cCCCGACCaacgcgcgugguUGGGGGAGGUgaCCaGGCGc- -3' miRNA: 3'- -GGGCUGG------------AUCUCCUCCA--GGaCUGCuu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 69395 | 0.69 | 0.729479 |
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Target: 5'- uUCGGcCCUGGAGGGGuUUCUGGCGGc -3' miRNA: 3'- gGGCU-GGAUCUCCUCcAGGACUGCUu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 21370 | 0.71 | 0.648451 |
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Target: 5'- gCUGGCCUccaugguAGAGGAGGccgUCCgGACGAu -3' miRNA: 3'- gGGCUGGA-------UCUCCUCC---AGGaCUGCUu -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 132916 | 0.72 | 0.548592 |
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Target: 5'- gCCGACCUcgGGGGGAGcGggCUGGCGGAc -3' miRNA: 3'- gGGCUGGA--UCUCCUC-CagGACUGCUU- -5' |
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| 5931 | 5' | -56.7 | NC_001806.1 | + | 79369 | 0.73 | 0.509445 |
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Target: 5'- -aCGGCCUGGAGGAGcucgcgUCUGACGAc -3' miRNA: 3'- ggGCUGGAUCUCCUCca----GGACUGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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