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miRNA: 3'- guCCGGCauAGGCa-GGCCgCCCGGCa -5'
miRNA: 3'- guCCGGCAUAGGCa---------GGCCG------CCCGGCa -5'
miRNA: 3'- guCCGGCaua-GGC------AGGCCGCCCGGCA- -5'
miRNA: 3'- -GUCCGGCauaGGCa-GGCC--GCCCGGCa -5'
miRNA: 3'- gUCCG-GCAUAgGCAGGCCGCCC-GGCa -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 5949 | 5' | -63.1 | NC_001806.1 | + | 147669 | 0.74 | 0.222261 |
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Target: 5'- -cGGCCGgcUCCGccCCGG-GGGCCGg -3' miRNA: 3'- guCCGGCauAGGCa-GGCCgCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 2221 | 0.74 | 0.207221 |
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Target: 5'- -cGGCCGUGUCCGgcccgcacagCCGGUuggccaGGGCCGc -3' miRNA: 3'- guCCGGCAUAGGCa---------GGCCG------CCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 124497 | 0.75 | 0.197689 |
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Target: 5'- -cGGUCGgacgCCGucucgcUCCGGCGGGCCGUc -3' miRNA: 3'- guCCGGCaua-GGC------AGGCCGCCCGGCA- -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 34411 | 0.75 | 0.18411 |
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Target: 5'- cCGGGCCGgg-CCGggCCGGgcCGGGCCGg -3' miRNA: 3'- -GUCCGGCauaGGCa-GGCC--GCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 64557 | 0.75 | 0.18411 |
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Target: 5'- aGGGCaUGUAUgCGUUCGGCGGGaCCGg -3' miRNA: 3'- gUCCG-GCAUAgGCAGGCCGCCC-GGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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