miRNA: 3'- -GUCCGGCa--UAGGCaGgCCGCC-CGGCA- -5'
miRNA: 3'- guCCG-GCAUAGg---CAGGCCGcC-CGGCa -5'
miRNA: 3'- gUCCGGCa--UAGGCaGGccGCCCGGCa -5'
miRNA: 3'- guCCGGCauaGGC-AGGCCG--CCCGGca -5'
miRNA: 3'- -GUCCGGCa-----UAGGcAGGCCgCCCGgCa -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5949 | 5' | -63.1 | NC_001806.1 | + | 49501 | 0.66 | 0.610113 |
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Target: 5'- gCGGGUCGgcgGUUCG-CgGGUGGcGCCGUc -3' miRNA: 3'- -GUCCGGCa--UAGGCaGgCCGCC-CGGCA- -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 69646 | 0.66 | 0.610113 |
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Target: 5'- -uGGCgCGUAUCauggGUCCGGCcGuGCCGa -3' miRNA: 3'- guCCG-GCAUAGg---CAGGCCGcC-CGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 20626 | 0.66 | 0.619821 |
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Target: 5'- gGGGCCGgggGUUCGaCCaaCGGGCCGc -3' miRNA: 3'- gUCCGGCa--UAGGCaGGccGCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 22421 | 0.66 | 0.619821 |
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Target: 5'- -cGGCCGg--CCGcUUCGGCugGGGCCu- -3' miRNA: 3'- guCCGGCauaGGC-AGGCCG--CCCGGca -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 56710 | 0.66 | 0.629538 |
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Target: 5'- cCGGGUCGcucuggGUCC-UCCGGgGGGCgGc -3' miRNA: 3'- -GUCCGGCa-----UAGGcAGGCCgCCCGgCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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