Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5949 | 5' | -63.1 | NC_001806.1 | + | 3325 | 0.68 | 0.457908 |
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Target: 5'- gGGGCCcgcggGUCCcUCCggccgcggggggcuGGCGGGCCGg -3' miRNA: 3'- gUCCGGca---UAGGcAGG--------------CCGCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 2926 | 0.72 | 0.29839 |
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Target: 5'- gCGGGCCGccgccUCCGggCGGcCGGGCCGg -3' miRNA: 3'- -GUCCGGCau---AGGCagGCC-GCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 2726 | 0.69 | 0.426097 |
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Target: 5'- gGGGUCGgggCCc-UCGGCGGGCCGg -3' miRNA: 3'- gUCCGGCauaGGcaGGCCGCCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 2221 | 0.74 | 0.207221 |
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Target: 5'- -cGGCCGUGUCCGgcccgcacagCCGGUuggccaGGGCCGc -3' miRNA: 3'- guCCGGCAUAGGCa---------GGCCG------CCCGGCa -5' |
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| 5949 | 5' | -63.1 | NC_001806.1 | + | 1463 | 0.67 | 0.518521 |
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Target: 5'- cCGGGCCGUcgUCGUCgccgcgcagcaccagCGGgGGGgCGUc -3' miRNA: 3'- -GUCCGGCAuaGGCAG---------------GCCgCCCgGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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