Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5953 | 3' | -49 | NC_001806.1 | + | 120611 | 0.66 | 0.998827 |
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Target: 5'- gCCUCUGUgGCAUagUUGucuuucCCGGCGGc- -3' miRNA: 3'- -GGAGAUAgCGUGaaAAC------GGCCGUUag -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 40085 | 0.66 | 0.998277 |
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Target: 5'- --gCUGUCGCAU--UUGgCGGCAAa- -3' miRNA: 3'- ggaGAUAGCGUGaaAACgGCCGUUag -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 60482 | 0.66 | 0.997929 |
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Target: 5'- cCCUCUGcCGC-CggccGCCGGCGGc- -3' miRNA: 3'- -GGAGAUaGCGuGaaaaCGGCCGUUag -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 84859 | 0.67 | 0.99519 |
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Target: 5'- gCCggcgGUCGCGCUccucugcgGCCGGCGGg- -3' miRNA: 3'- -GGaga-UAGCGUGAaaa-----CGGCCGUUag -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 149788 | 0.68 | 0.990438 |
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Target: 5'- cCCUCaccUCGCGCUgggggcggcccucagGCCGGCGGg- -3' miRNA: 3'- -GGAGau-AGCGUGAaaa------------CGGCCGUUag -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 132463 | 0.68 | 0.988622 |
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Target: 5'- gCUC-GUCGCcCUUUU-CCGGCAGUg -3' miRNA: 3'- gGAGaUAGCGuGAAAAcGGCCGUUAg -5' |
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| 5953 | 3' | -49 | NC_001806.1 | + | 33086 | 0.71 | 0.948646 |
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Target: 5'- gCCUUUuUCGCGuCgcgUGCCGGCGAg- -3' miRNA: 3'- -GGAGAuAGCGU-GaaaACGGCCGUUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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