Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5954 | 3' | -55.3 | NC_001806.1 | + | 133822 | 0.67 | 0.88774 |
Target: 5'- cCGGGGUGCUGGCGggaguaUGGgGCGAGggGg -3' miRNA: 3'- uGCUCUGCGACCGC------ACCaCGUUCuaC- -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 143261 | 0.67 | 0.894528 |
Target: 5'- -gGGGAUGUgGGCGggGGUGCGcgggAGGUGu -3' miRNA: 3'- ugCUCUGCGaCCGCa-CCACGU----UCUAC- -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 148190 | 0.66 | 0.91931 |
Target: 5'- cGCGGGGCGUccGGCGgGGgGCGGGcgGu -3' miRNA: 3'- -UGCUCUGCGa-CCGCaCCaCGUUCuaC- -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 151200 | 0.7 | 0.732381 |
Target: 5'- gGCGAGGCGCggcggaaGGCGgaaggGGcGCGAGggGg -3' miRNA: 3'- -UGCUCUGCGa------CCGCa----CCaCGUUCuaC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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