Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5954 | 3' | -55.3 | NC_001806.1 | + | 11307 | 0.67 | 0.894528 |
Target: 5'- -gGAGGCGUUGGgGUGGgugucGUggGGUc -3' miRNA: 3'- ugCUCUGCGACCgCACCa----CGuuCUAc -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 10405 | 0.66 | 0.930246 |
Target: 5'- gGCGGGAgccgggggguccCGCUGGCG-GGagugccgGCGAGAc- -3' miRNA: 3'- -UGCUCU------------GCGACCGCaCCa------CGUUCUac -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 5664 | 0.66 | 0.9249 |
Target: 5'- gGCGGGGCGCgagGGCG-GGUGgGu---- -3' miRNA: 3'- -UGCUCUGCGa--CCGCaCCACgUucuac -5' |
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5954 | 3' | -55.3 | NC_001806.1 | + | 3288 | 0.7 | 0.742257 |
Target: 5'- gGCGGGGCGCgucGGCGUGcGgcggcgGCGGGGa- -3' miRNA: 3'- -UGCUCUGCGa--CCGCAC-Ca-----CGUUCUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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