Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5955 | 5' | -50.3 | NC_001806.1 | + | 136945 | 0.67 | 0.985958 |
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Target: 5'- cGUGUCCCCCCgc--GCACgcguaGugGGg -3' miRNA: 3'- -CGUAGGGGGGaaaaCGUGaua--CugUC- -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 139058 | 0.67 | 0.985958 |
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Target: 5'- -aGUCgCCCCCUUUaGCACguacgugGACAa -3' miRNA: 3'- cgUAG-GGGGGAAAaCGUGaua----CUGUc -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 119103 | 0.67 | 0.984129 |
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Target: 5'- uCGUCCCCCCcaacacGCGCUAcgcggccGACAGc -3' miRNA: 3'- cGUAGGGGGGaaaa--CGUGAUa------CUGUC- -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 123192 | 0.67 | 0.983742 |
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Target: 5'- aGCA-CCCCCCgggucgcccGCAggGUGGCGGa -3' miRNA: 3'- -CGUaGGGGGGaaaa-----CGUgaUACUGUC- -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 80710 | 0.68 | 0.977552 |
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Target: 5'- gGCAUCCUCCCUc--GCGCUAcacaucGACGc -3' miRNA: 3'- -CGUAGGGGGGAaaaCGUGAUa-----CUGUc -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 79339 | 0.68 | 0.972172 |
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Target: 5'- cCGUCCCCCCUc--GCAUgugGGCAu -3' miRNA: 3'- cGUAGGGGGGAaaaCGUGauaCUGUc -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 29839 | 0.68 | 0.972172 |
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Target: 5'- cGCAgCCCCCCagccgGCcgcgGCUcgGACAGc -3' miRNA: 3'- -CGUaGGGGGGaaaa-CG----UGAuaCUGUC- -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 121839 | 0.72 | 0.894337 |
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Target: 5'- aCAUCCCCCCgaccaGCGCcggGUGGCGc -3' miRNA: 3'- cGUAGGGGGGaaaa-CGUGa--UACUGUc -5' |
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| 5955 | 5' | -50.3 | NC_001806.1 | + | 30575 | 0.74 | 0.80434 |
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Target: 5'- cGCGUCCCCCCcuc-GCGCc--GGCAGg -3' miRNA: 3'- -CGUAGGGGGGaaaaCGUGauaCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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