Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5958 | 3' | -44.9 | NC_001806.1 | + | 141274 | 0.68 | 0.999935 |
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Target: 5'- gGUAUCgGUUCgccaCGAGGAaAUGCUGGc -3' miRNA: 3'- -CAUAGgCAAGa---GUUUUUgUACGACCu -5' |
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| 5958 | 3' | -44.9 | NC_001806.1 | + | 138041 | 0.68 | 0.999913 |
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Target: 5'- -aGUUCGguaacCUCAuGAAgGUGCUGGAg -3' miRNA: 3'- caUAGGCaa---GAGUuUUUgUACGACCU- -5' |
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| 5958 | 3' | -44.9 | NC_001806.1 | + | 20497 | 0.69 | 0.999803 |
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Target: 5'- ---cCCGUUcCUCGuuAGCAUGC-GGAa -3' miRNA: 3'- cauaGGCAA-GAGUuuUUGUACGaCCU- -5' |
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| 5958 | 3' | -44.9 | NC_001806.1 | + | 62724 | 0.69 | 0.999586 |
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Target: 5'- --uUCCaGUUCcuuuagCAGGAGCGUGUUGGGg -3' miRNA: 3'- cauAGG-CAAGa-----GUUUUUGUACGACCU- -5' |
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| 5958 | 3' | -44.9 | NC_001806.1 | + | 34244 | 0.69 | 0.999478 |
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Target: 5'- cGUGUCUGUUCUU----GCGUgugGCUGGGg -3' miRNA: 3'- -CAUAGGCAAGAGuuuuUGUA---CGACCU- -5' |
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| 5958 | 3' | -44.9 | NC_001806.1 | + | 73357 | 0.78 | 0.894105 |
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Target: 5'- cGUAUCCGgccUCUCcGGGGC-UGCUGGAg -3' miRNA: 3'- -CAUAGGCa--AGAGuUUUUGuACGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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