Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5965 | 5' | -54.2 | NC_001806.1 | + | 90254 | 0.66 | 0.947493 |
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Target: 5'- gUCGCCGUGgcgCCC--CUGGUCguggGCCu -3' miRNA: 3'- -AGCGGUACaaaGGGcaGACCAGg---UGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 93939 | 0.66 | 0.946624 |
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Target: 5'- aCGCCggGUagcguacggaCCGcCUcGGUCCACCg -3' miRNA: 3'- aGCGGuaCAaag-------GGCaGA-CCAGGUGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 99913 | 0.66 | 0.946186 |
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Target: 5'- gCGCCGUGauggUCUCG-UUGGUCCcgggagaauggcggGCCg -3' miRNA: 3'- aGCGGUACaa--AGGGCaGACCAGG--------------UGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 87442 | 0.67 | 0.943509 |
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Target: 5'- aUCGCCccuggGUGUccggcagaauaaagCCCuUCUGGUCgGCCa -3' miRNA: 3'- -AGCGG-----UACAaa------------GGGcAGACCAGgUGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 144758 | 0.67 | 0.943054 |
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Target: 5'- gUCGCCGgaaUGUUUCguUCGUCU-GUCC-CCu -3' miRNA: 3'- -AGCGGU---ACAAAG--GGCAGAcCAGGuGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 62198 | 0.67 | 0.938378 |
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Target: 5'- aUCGCCAgGUccgccgUCgCGcUCUGGUCCguaaggGCCg -3' miRNA: 3'- -AGCGGUaCAa-----AGgGC-AGACCAGG------UGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 90890 | 0.67 | 0.928308 |
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Target: 5'- aCGCCGUGg--CCCGcgcGG-CCGCCc -3' miRNA: 3'- aGCGGUACaaaGGGCagaCCaGGUGG- -5' |
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| 5965 | 5' | -54.2 | NC_001806.1 | + | 146879 | 1.13 | 0.002446 |
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Target: 5'- gUCGCCAUGUUUCCCGUCUGGUCCACCa -3' miRNA: 3'- -AGCGGUACAAAGGGCAGACCAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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