Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5971 | 3' | -54 | NC_001806.1 | + | 136900 | 0.69 | 0.859083 |
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Target: 5'- gGGGUGAAccuuuacCCAGCcguccucgggGGAGCACaGCGCu -3' miRNA: 3'- -UCCACUUu------GGUUG----------UCUCGUGcCGCGu -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 142167 | 0.71 | 0.741634 |
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Target: 5'- gGGGUGggGuCCGACGuggcgaugauGGGCGgCGGCGUg -3' miRNA: 3'- -UCCACuuU-GGUUGU----------CUCGU-GCCGCGu -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 143364 | 0.71 | 0.741634 |
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Target: 5'- gAGGgacgGggGCCGGCAGAcCgACGGCGaCAa -3' miRNA: 3'- -UCCa---CuuUGGUUGUCUcG-UGCCGC-GU- -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 144216 | 0.67 | 0.902181 |
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Target: 5'- ---cGAcACC-GCAGAGC-CGGCGCGc -3' miRNA: 3'- uccaCUuUGGuUGUCUCGuGCCGCGU- -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 147622 | 0.71 | 0.761332 |
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Target: 5'- cAGGgacGAGugCGACuGGGGCacACGGCGCGc -3' miRNA: 3'- -UCCa--CUUugGUUG-UCUCG--UGCCGCGU- -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 151181 | 0.68 | 0.895585 |
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Target: 5'- cGGUGAcGCgcaGGCGGAGgGCgaGGCGCGg -3' miRNA: 3'- uCCACUuUGg--UUGUCUCgUG--CCGCGU- -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 151278 | 0.68 | 0.895585 |
|
Target: 5'- cGGUgGggGCCGGggcCGGGGgGCGGCgGCGg -3' miRNA: 3'- uCCA-CuuUGGUU---GUCUCgUGCCG-CGU- -5' |
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| 5971 | 3' | -54 | NC_001806.1 | + | 151432 | 0.69 | 0.825993 |
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Target: 5'- gGGGgcccACCGGCGGGGgGCGGCgGCGg -3' miRNA: 3'- -UCCacuuUGGUUGUCUCgUGCCG-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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