Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5972 | 5' | -52.4 | NC_001806.1 | + | 129197 | 0.66 | 0.978118 |
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Target: 5'- cGUCUCcugucGUCGGAGuuuuacCCAGACCCUa -3' miRNA: 3'- -UAGAGuc---CAGUCUCua----GGUUUGGGAg -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 2377 | 0.66 | 0.978118 |
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Target: 5'- -cCUCgGGGUCGGGGAucuggcgcaUCCAGGCCg-- -3' miRNA: 3'- uaGAG-UCCAGUCUCU---------AGGUUUGGgag -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 12911 | 0.66 | 0.978118 |
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Target: 5'- ----gGGGUCcuccaAGAGggCCGAAUCCUCg -3' miRNA: 3'- uagagUCCAG-----UCUCuaGGUUUGGGAG- -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 74048 | 0.66 | 0.975596 |
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Target: 5'- -gUUCAGGUUcggcuggaGGAGGUgCGGACCCa- -3' miRNA: 3'- uaGAGUCCAG--------UCUCUAgGUUUGGGag -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 86354 | 0.66 | 0.975596 |
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Target: 5'- -cCUCGGG-CAGGGGUCgCGccAGCCaCUCc -3' miRNA: 3'- uaGAGUCCaGUCUCUAG-GU--UUGG-GAG- -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 16869 | 0.66 | 0.975596 |
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Target: 5'- gGUCUCcGGgggCGGGGAgUCCAGGCacgcguCCUCg -3' miRNA: 3'- -UAGAGuCCa--GUCUCU-AGGUUUG------GGAG- -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 19026 | 0.68 | 0.937934 |
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Target: 5'- ---cCAGGUCGGGGGUCCAucguGCgCUg -3' miRNA: 3'- uagaGUCCAGUCUCUAGGUu---UGgGAg -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 5008 | 0.68 | 0.93281 |
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Target: 5'- gGUCgu-GGUCGGGGucGUCCccGCCCUCc -3' miRNA: 3'- -UAGaguCCAGUCUC--UAGGuuUGGGAG- -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 92128 | 0.68 | 0.915886 |
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Target: 5'- uUCUCGGG-CGGcGAUCCAa--CCUCg -3' miRNA: 3'- uAGAGUCCaGUCuCUAGGUuugGGAG- -5' |
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| 5972 | 5' | -52.4 | NC_001806.1 | + | 790 | 1.07 | 0.007112 |
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Target: 5'- aAUCUCAGGUCAGAGAUCCAAACCCUCc -3' miRNA: 3'- -UAGAGUCCAGUCUCUAGGUUUGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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