Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5978 | 5' | -61.3 | NC_001806.1 | + | 4961 | 0.67 | 0.664352 |
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Target: 5'- gGGCCc--UCCCgUCCCGCcggGCgUCGUCg -3' miRNA: 3'- -CCGGcuaGGGGgAGGGCG---CGaAGCAG- -5' |
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| 5978 | 5' | -61.3 | NC_001806.1 | + | 23105 | 0.67 | 0.664352 |
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Target: 5'- cGCCGGggCCCUgggcCCCGCGCUgccgCGg- -3' miRNA: 3'- cCGGCUagGGGGa---GGGCGCGAa---GCag -5' |
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| 5978 | 5' | -61.3 | NC_001806.1 | + | 142062 | 0.67 | 0.664352 |
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Target: 5'- cGGCCGGUucgcCCCCCgaaCCCGgacggGUUUcCGUCg -3' miRNA: 3'- -CCGGCUA----GGGGGa--GGGCg----CGAA-GCAG- -5' |
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| 5978 | 5' | -61.3 | NC_001806.1 | + | 993 | 0.66 | 0.674106 |
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Target: 5'- cGCCcccgCCCCCUCCCGCcCcUCGcCc -3' miRNA: 3'- cCGGcua-GGGGGAGGGCGcGaAGCaG- -5' |
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| 5978 | 5' | -61.3 | NC_001806.1 | + | 1145 | 0.66 | 0.674106 |
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Target: 5'- cGCCccucgCCCCCUCCCGCcCcUCGa- -3' miRNA: 3'- cCGGcua--GGGGGAGGGCGcGaAGCag -5' |
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| 5978 | 5' | -61.3 | NC_001806.1 | + | 71220 | 0.66 | 0.722244 |
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Target: 5'- cGGCgUGuuUCgCgCUUCCGCGUUUCGUCc -3' miRNA: 3'- -CCG-GCu-AGgGgGAGGGCGCGAAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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