Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5985 | 5' | -58.4 | NC_001806.1 | + | 19947 | 0.66 | 0.821867 |
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Target: 5'- gGUG-GGCGgGUCUUCCCCCc----- -3' miRNA: 3'- gCACaCCGCgUAGAGGGGGGcacuau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 3509 | 0.66 | 0.813335 |
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Target: 5'- gCGUGgcgGGCgGCGUCggggucgucgCCCCCCGcGGg- -3' miRNA: 3'- -GCACa--CCG-CGUAGa---------GGGGGGCaCUau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 121824 | 0.67 | 0.759057 |
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Target: 5'- gGUGcGGCGCGgacaacaUCCCCCCGa---- -3' miRNA: 3'- gCACaCCGCGUag-----AGGGGGGCacuau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 144327 | 0.67 | 0.720563 |
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Target: 5'- cCGUGUGG-GUAUCcggCCCCCCGc---- -3' miRNA: 3'- -GCACACCgCGUAGa--GGGGGGCacuau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 119587 | 0.69 | 0.660773 |
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Target: 5'- gGUGcUGGCGgGUCUggagCCCCCCGgcgGGg- -3' miRNA: 3'- gCAC-ACCGCgUAGA----GGGGGGCa--CUau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 104040 | 0.69 | 0.630459 |
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Target: 5'- --aGUGGCGCAgcugCUUcaUCCCCGUGGc- -3' miRNA: 3'- gcaCACCGCGUa---GAG--GGGGGCACUau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 29800 | 0.69 | 0.620347 |
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Target: 5'- ----aGGCGCcgCcgcgCCCCCCGUGAc- -3' miRNA: 3'- gcacaCCGCGuaGa---GGGGGGCACUau -5' |
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| 5985 | 5' | -58.4 | NC_001806.1 | + | 84899 | 0.7 | 0.600156 |
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Target: 5'- --cGUGGUGCGUCgggCCUCCCG-GAa- -3' miRNA: 3'- gcaCACCGCGUAGa--GGGGGGCaCUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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