miRNA: 3'- -CGGCUGG----ACAUAUG-Gau-AGa--CUCAUC- -5'
miRNA: 3'- -CGGCUGGACAuaUGGauagaCUCAUc -5'
Results 1 - 2 of 2 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5987 | 3' | -48.6 | NC_001806.1 | + | 73732 | 0.66 | 0.99872 |
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Target: 5'- cGCCGGCCaaucUGUAUACaCgccUCggcGAGUGGc -3' miRNA: 3'- -CGGCUGG----ACAUAUG-Gau-AGa--CUCAUC- -5' |
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| 5987 | 3' | -48.6 | NC_001806.1 | + | 47420 | 0.69 | 0.988012 |
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Target: 5'- cGCCGACCUGU--ACCgg---GAGUGu -3' miRNA: 3'- -CGGCUGGACAuaUGGauagaCUCAUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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