Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5989 | 5' | -51 | NC_001806.1 | + | 115075 | 0.75 | 0.726151 |
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Target: 5'- -gCGGGAaaccgccuGGGGCcGGCCCAGCCcGUCCc -3' miRNA: 3'- caGCUCU--------UCCUGuUUGGGUUGG-CAGG- -5' |
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| 5989 | 5' | -51 | NC_001806.1 | + | 56682 | 0.75 | 0.723111 |
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Target: 5'- gGUCGGGggGGACGggGGCCCGggaACCccgggucgcucuggGUCCu -3' miRNA: 3'- -CAGCUCuuCCUGU--UUGGGU---UGG--------------CAGG- -5' |
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| 5989 | 5' | -51 | NC_001806.1 | + | 132559 | 0.76 | 0.705753 |
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Target: 5'- -cCGGGggGGACccguCCCGuacuuuGCCGUCCu -3' miRNA: 3'- caGCUCuuCCUGuuu-GGGU------UGGCAGG- -5' |
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| 5989 | 5' | -51 | NC_001806.1 | + | 136892 | 0.79 | 0.559771 |
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Target: 5'- -cCGGGGcugGGGugAAccuuuACCCAGCCGUCCu -3' miRNA: 3'- caGCUCU---UCCugUU-----UGGGUUGGCAGG- -5' |
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| 5989 | 5' | -51 | NC_001806.1 | + | 49557 | 0.83 | 0.338779 |
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Target: 5'- -aCGAGGAGGACGAcuACCCGgaaGCCGUCg -3' miRNA: 3'- caGCUCUUCCUGUU--UGGGU---UGGCAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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