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miRNA: 3'- aGGCCCGGa-------GUCUUGGgUGGGCU--UUG- -5'
miRNA: 3'- aGGCCCGGAG-UCUUggGUGGGCUUUG- -5'
miRNA: 3'- -AGGCCCGGAGUCu-UGGGUGGGCuuUG- -5'
Results 141 - 143 of 143 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 5990 | 3' | -57.6 | NC_001806.1 | + | 27080 | 0.77 | 0.267908 |
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Target: 5'- cCCGGGCCcuggcccgCGGAGCCgGCCCGGcgAACu -3' miRNA: 3'- aGGCCCGGa-------GUCUUGGgUGGGCU--UUG- -5' |
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| 5990 | 3' | -57.6 | NC_001806.1 | + | 22119 | 0.8 | 0.195937 |
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Target: 5'- cCCGGGCCUCuGGGGggCGCCCGAGGCg -3' miRNA: 3'- aGGCCCGGAG-UCUUggGUGGGCUUUG- -5' |
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| 5990 | 3' | -57.6 | NC_001806.1 | + | 26947 | 0.83 | 0.112239 |
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Target: 5'- gUCUGGGCCUCGGccGCCCGCCUGgcGCg -3' miRNA: 3'- -AGGCCCGGAGUCu-UGGGUGGGCuuUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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