Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5991 | 3' | -51.9 | NC_001806.1 | + | 132172 | 0.66 | 0.977934 |
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Target: 5'- cGACGCg--CGGCUGGCGUGGg----- -3' miRNA: 3'- -CUGUGgugGUCGACCGUACCauauuu -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 51283 | 0.66 | 0.972567 |
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Target: 5'- aGCucCCugCGGCUGGCGcGGUGc--- -3' miRNA: 3'- cUGu-GGugGUCGACCGUaCCAUauuu -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 2798 | 0.66 | 0.966316 |
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Target: 5'- gGGCGCCGCgGGCUgGGCggGGg----- -3' miRNA: 3'- -CUGUGGUGgUCGA-CCGuaCCauauuu -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 38625 | 0.67 | 0.962841 |
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Target: 5'- -cCACCACCGGCcGG-GUGGUGg--- -3' miRNA: 3'- cuGUGGUGGUCGaCCgUACCAUauuu -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 108189 | 0.67 | 0.959123 |
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Target: 5'- --gGCCGCCucuGCgcugGGCGUGGuUAUGAu -3' miRNA: 3'- cugUGGUGGu--CGa---CCGUACC-AUAUUu -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 16832 | 0.68 | 0.941725 |
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Target: 5'- cGGCcUCGCUGGgUGGUGUGGUGUGGGa -3' miRNA: 3'- -CUGuGGUGGUCgACCGUACCAUAUUU- -5' |
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| 5991 | 3' | -51.9 | NC_001806.1 | + | 34722 | 0.71 | 0.793869 |
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Target: 5'- cGCACCACCAGCacGGguUGGggGUAGc -3' miRNA: 3'- cUGUGGUGGUCGa-CCguACCa-UAUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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