Results 121 - 126 of 126 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5996 | 5' | -60.1 | NC_001806.1 | + | 151440 | 0.66 | 0.795638 |
Target: 5'- aCCGGCgggggGCGgCGgcgggGCgGCCGCGGGcGCGc -3' miRNA: 3'- -GGCUG-----CGCgGCa----CG-CGGCGCUCaUGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 3604 | 0.66 | 0.795638 |
Target: 5'- cCCGGCGgGCacCGcGCGCuCGCccGGUGCGg -3' miRNA: 3'- -GGCUGCgCG--GCaCGCG-GCGc-UCAUGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 54928 | 0.66 | 0.795638 |
Target: 5'- gUCGGgGUGCuCGUGCGuCUGCGuGU-CGa -3' miRNA: 3'- -GGCUgCGCG-GCACGC-GGCGCuCAuGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 23712 | 0.66 | 0.804183 |
Target: 5'- aUCGcCGCGCgGUGCGCCGgGcccGCc -3' miRNA: 3'- -GGCuGCGCGgCACGCGGCgCucaUGc -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 122516 | 0.66 | 0.804183 |
Target: 5'- uCCaGCGUGCCGUacGUccgGCCGUGGGUGg- -3' miRNA: 3'- -GGcUGCGCGGCA--CG---CGGCGCUCAUgc -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 136370 | 0.66 | 0.804183 |
Target: 5'- gCCGGCGCGCUccGCGgccuCCGCGAccGUGg- -3' miRNA: 3'- -GGCUGCGCGGcaCGC----GGCGCU--CAUgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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