Results 121 - 126 of 126 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5996 | 5' | -60.1 | NC_001806.1 | + | 2787 | 0.71 | 0.474784 |
Target: 5'- gCGGCG-GCCcgggGCGCCGCGGGcuggGCGg -3' miRNA: 3'- gGCUGCgCGGca--CGCGGCGCUCa---UGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 2504 | 0.66 | 0.786958 |
Target: 5'- gCCGcACGCgGCC-UGgGCgGCGGGgGCGg -3' miRNA: 3'- -GGC-UGCG-CGGcACgCGgCGCUCaUGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 1780 | 0.7 | 0.567204 |
Target: 5'- aCCGGCGUGUccgggccgaagcgCGUGCGCaCGCGGuaGCGc -3' miRNA: 3'- -GGCUGCGCG-------------GCACGCG-GCGCUcaUGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 1678 | 0.75 | 0.308414 |
Target: 5'- uCCGGCGCGCCGgGCGCCaugGCGucGGUGg- -3' miRNA: 3'- -GGCUGCGCGGCaCGCGG---CGC--UCAUgc -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 1616 | 0.67 | 0.723089 |
Target: 5'- gCGGCGCGCCcaggccccagcGcGCGCagGCGGcGUGCGa -3' miRNA: 3'- gGCUGCGCGG-----------CaCGCGg-CGCU-CAUGC- -5' |
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5996 | 5' | -60.1 | NC_001806.1 | + | 356 | 0.66 | 0.795638 |
Target: 5'- cCCGccuuuuuuGCGCGCgCGcGCGCCcGCGGGggGCc -3' miRNA: 3'- -GGC--------UGCGCG-GCaCGCGG-CGCUCa-UGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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