Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5999 | 3' | -57.8 | NC_001806.1 | + | 126679 | 0.66 | 0.798021 |
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Target: 5'- --cGUGUCCuGGAgcuccGGGgaucugGUCCGCCGc -3' miRNA: 3'- uacCACAGGuCCU-----UCCa-----CAGGCGGUa -5' |
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| 5999 | 3' | -57.8 | NC_001806.1 | + | 91395 | 0.66 | 0.770214 |
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Target: 5'- cGUGGUGcCCGGGuucGAGG-GUCgGCCc- -3' miRNA: 3'- -UACCACaGGUCC---UUCCaCAGgCGGua -5' |
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| 5999 | 3' | -57.8 | NC_001806.1 | + | 125249 | 0.67 | 0.740304 |
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Target: 5'- aAUGGaGUCCAcGGuAGGcccagcaUGUCCGCCGg -3' miRNA: 3'- -UACCaCAGGU-CCuUCC-------ACAGGCGGUa -5' |
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| 5999 | 3' | -57.8 | NC_001806.1 | + | 82140 | 0.67 | 0.711484 |
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Target: 5'- gAUGGUGUCCAGGucgccGAGGgacagccCCGUCGUc -3' miRNA: 3'- -UACCACAGGUCC-----UUCCaca----GGCGGUA- -5' |
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| 5999 | 3' | -57.8 | NC_001806.1 | + | 121698 | 0.7 | 0.547902 |
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Target: 5'- cUGGcgGUCCAGGggGaugcuagccuGUGUCCGCUu- -3' miRNA: 3'- uACCa-CAGGUCCuuC----------CACAGGCGGua -5' |
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| 5999 | 3' | -57.8 | NC_001806.1 | + | 62477 | 0.71 | 0.479232 |
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Target: 5'- -gGGUGgaggCUGGGAGGGUcccGUCCGCCu- -3' miRNA: 3'- uaCCACa---GGUCCUUCCA---CAGGCGGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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