miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
6005 5' -55.3 NC_001809.1 + 9122 0.66 0.228445
Target:  5'- aCUCCAGgaagcgGCUccccAGCCACAUguaccaGAUGGCc- -3'
miRNA:   3'- -GAGGUCa-----CGA----UCGGUGUG------UUGCCGcu -5'
6005 5' -55.3 NC_001809.1 + 5380 0.71 0.095154
Target:  5'- cCUCCAGccUGUUGGUCACugAcuGCuGGCGAa -3'
miRNA:   3'- -GAGGUC--ACGAUCGGUGugU--UG-CCGCU- -5'
6005 5' -55.3 NC_001809.1 + 919 1.08 0.000096
Target:  5'- uCUCCAGUGCUAGCCACACAACGGCGAc -3'
miRNA:   3'- -GAGGUCACGAUCGGUGUGUUGCCGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.