Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6008 | 3' | -48.3 | NC_001809.1 | + | 7529 | 0.66 | 0.591433 |
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Target: 5'- -uGUCCGGUgGccGCCAgcccCACaGCCGCa -3' miRNA: 3'- auUAGGUCAgUuuUGGUa---GUG-CGGUG- -5' |
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| 6008 | 3' | -48.3 | NC_001809.1 | + | 8702 | 0.67 | 0.513607 |
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Target: 5'- ---gUCAGUCAugGCCAUgCGCaCCACa -3' miRNA: 3'- auuaGGUCAGUuuUGGUA-GUGcGGUG- -5' |
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| 6008 | 3' | -48.3 | NC_001809.1 | + | 957 | 0.69 | 0.428654 |
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Target: 5'- ---cCCGGUCuacGCC-UCACGCUGCa -3' miRNA: 3'- auuaGGUCAGuuuUGGuAGUGCGGUG- -5' |
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| 6008 | 3' | -48.3 | NC_001809.1 | + | 6295 | 0.69 | 0.38398 |
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Target: 5'- -uGUCCAGcuUCGGu-CCGUCACGCUAg -3' miRNA: 3'- auUAGGUC--AGUUuuGGUAGUGCGGUg -5' |
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| 6008 | 3' | -48.3 | NC_001809.1 | + | 7681 | 0.7 | 0.342505 |
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Target: 5'- --cUCCA-UCAGAGCCAcCACGCCu- -3' miRNA: 3'- auuAGGUcAGUUUUGGUaGUGCGGug -5' |
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| 6008 | 3' | -48.3 | NC_001809.1 | + | 5674 | 1.09 | 0.000508 |
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Target: 5'- gUAAUCCAGUCAAAACCAUCACGCCACu -3' miRNA: 3'- -AUUAGGUCAGUUUUGGUAGUGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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