Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 601 | 5' | -53.7 | AC_000017.1 | + | 16380 | 0.66 | 0.685654 |
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Target: 5'- cCUGGaccuggGGGGCACAGUGacaauacccGcGGCCa -3' miRNA: 3'- -GACCaaauuaCCCCGUGUCGC---------C-CUGG- -5' |
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| 601 | 5' | -53.7 | AC_000017.1 | + | 22246 | 0.66 | 0.674267 |
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Target: 5'- -aGGUUcaUGGUGGGGUugugauccaugGgAGUuuGGGGCCa -3' miRNA: 3'- gaCCAA--AUUACCCCG-----------UgUCG--CCCUGG- -5' |
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| 601 | 5' | -53.7 | AC_000017.1 | + | 23937 | 0.66 | 0.658254 |
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Target: 5'- uUGGgggcgcgcGGGGgGCGGCGGcGACg -3' miRNA: 3'- gACCaaauua--CCCCgUGUCGCC-CUGg -5' |
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| 601 | 5' | -53.7 | AC_000017.1 | + | 15732 | 0.69 | 0.515385 |
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Target: 5'- aUGGUg----GGGGC-UGGCGGGcgcGCCa -3' miRNA: 3'- gACCAaauuaCCCCGuGUCGCCC---UGG- -5' |
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| 601 | 5' | -53.7 | AC_000017.1 | + | 9415 | 0.71 | 0.364566 |
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Target: 5'- gUGGgggAggGGGGaCACGGCGGcGACg -3' miRNA: 3'- gACCaaaUuaCCCC-GUGUCGCC-CUGg -5' |
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| 601 | 5' | -53.7 | AC_000017.1 | + | 1474 | 1.13 | 0.00039 |
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Target: 5'- aCUGGUUUAAUGGGGCACAGCGGGACCa -3' miRNA: 3'- -GACCAAAUUACCCCGUGUCGCCCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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