miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
6019 5' -52.5 NC_001809.1 + 6471 0.66 0.427574
Target:  5'- cCGAA----GCUGCGACGcccAGACGCGu -3'
miRNA:   3'- aGCUUucucUGGCGCUGCu--UCUGCGC- -5'
6019 5' -52.5 NC_001809.1 + 7479 0.66 0.406212
Target:  5'- aUGAcAGAGGCUGCGGCuguGGGGCugGCGg -3'
miRNA:   3'- aGCUuUCUCUGGCGCUGc--UUCUG--CGC- -5'
6019 5' -52.5 NC_001809.1 + 3584 0.68 0.336971
Target:  5'- uUCGGAGGAGACCuucuacaggaGUGACGGucguauggGGAgGUGu -3'
miRNA:   3'- -AGCUUUCUCUGG----------CGCUGCU--------UCUgCGC- -5'
6019 5' -52.5 NC_001809.1 + 183 1.09 0.000234
Target:  5'- gUCGAAAGAGACCGCGACGAAGACGCGu -3'
miRNA:   3'- -AGCUUUCUCUGGCGCUGCUUCUGCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.