Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
603 | 3' | -58.8 | AC_000017.1 | + | 17049 | 0.65 | 0.426355 |
Target: 5'- gGCGCCCaccgugcagcucauGGUGC-CCaaGCgccaACGACUGGa -3' miRNA: 3'- -UGCGGG--------------UCGCGaGGa-CGa---UGCUGACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 24014 | 0.66 | 0.423474 |
Target: 5'- cGCGUCC-GCGCUCgggggugguuucgcgCUGCUccucuuccCGACUGGc -3' miRNA: 3'- -UGCGGGuCGCGAG---------------GACGAu-------GCUGACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 16153 | 0.66 | 0.410184 |
Target: 5'- -aGCCCGGCGCUa-UGCUaaaaugaagagACGGC-GGa -3' miRNA: 3'- ugCGGGUCGCGAggACGA-----------UGCUGaCC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 26098 | 0.66 | 0.410184 |
Target: 5'- cCGCCaAGaGUUUCUGCUACGAaaGGg -3' miRNA: 3'- uGCGGgUCgCGAGGACGAUGCUgaCC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 8924 | 0.66 | 0.400851 |
Target: 5'- cGCGUCCGGCucGCUCCacgGUgGCGGCgaGGu -3' miRNA: 3'- -UGCGGGUCG--CGAGGa--CGaUGCUGa-CC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 34134 | 0.66 | 0.400851 |
Target: 5'- uACGUCCGGCGUUCCauuugGC-AUGACa-- -3' miRNA: 3'- -UGCGGGUCGCGAGGa----CGaUGCUGacc -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 18398 | 0.67 | 0.37368 |
Target: 5'- cACGgCCGGUggagGCUCCU-CUACGGgaGGg -3' miRNA: 3'- -UGCgGGUCG----CGAGGAcGAUGCUgaCC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 16053 | 0.67 | 0.356274 |
Target: 5'- cGCGCCCcaggGGCGCgguagugCCcGCgcacGCGcACUGGg -3' miRNA: 3'- -UGCGGG----UCGCGa------GGaCGa---UGC-UGACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 26917 | 0.67 | 0.339445 |
Target: 5'- gGCGCCagacgCAGCGCUCCUccucCUGCuGCUGc -3' miRNA: 3'- -UGCGG-----GUCGCGAGGAc---GAUGcUGACc -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 12997 | 0.67 | 0.331249 |
Target: 5'- gACGCCCAacuCGCgCCUGUUGCuGCUGc -3' miRNA: 3'- -UGCGGGUc--GCGaGGACGAUGcUGACc -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 10113 | 0.68 | 0.288064 |
Target: 5'- cGCGCUCGGCuaauauggCCUGCUGCaccugcgugaggguaGACUGGa -3' miRNA: 3'- -UGCGGGUCGcga-----GGACGAUG---------------CUGACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 17219 | 0.69 | 0.270956 |
Target: 5'- cACGCCCAGUcccgguGCcaCCUGCU-UGAUUGGu -3' miRNA: 3'- -UGCGGGUCG------CGa-GGACGAuGCUGACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 19213 | 0.69 | 0.250733 |
Target: 5'- -aGCCCccaaaGGCGCUCCcaauucCUGCGAgUGGg -3' miRNA: 3'- ugCGGG-----UCGCGAGGac----GAUGCUgACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 18859 | 0.7 | 0.225724 |
Target: 5'- gGCGaCUCAGCaGCUCCUcugGCgGCGACaUGGa -3' miRNA: 3'- -UGC-GGGUCG-CGAGGA---CGaUGCUG-ACC- -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 2232 | 0.72 | 0.167683 |
Target: 5'- cCGCCUGGCuucCUCCUGCUGCuGCUGu -3' miRNA: 3'- uGCGGGUCGc--GAGGACGAUGcUGACc -5' |
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603 | 3' | -58.8 | AC_000017.1 | + | 4348 | 1.1 | 0.000212 |
Target: 5'- cACGCCCAGCGCUCCUGCUACGACUGGa -3' miRNA: 3'- -UGCGGGUCGCGAGGACGAUGCUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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