Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6033 | 3' | -46.9 | NC_001813.1 | + | 27696 | 0.66 | 0.963275 |
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Target: 5'- uUCACAGUUAUcgucaucuaaaucacAGUUCAuuGCUCCcAACu -3' miRNA: 3'- -AGUGUCGAUA---------------UCAAGU--UGGGGuUUGc -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 27891 | 0.66 | 0.947805 |
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Target: 5'- -uGguGCUGUGGggCAAUCUCAggUGg -3' miRNA: 3'- agUguCGAUAUCaaGUUGGGGUuuGC- -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 13735 | 0.67 | 0.94254 |
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Target: 5'- cUCACaAGUUAgGGcccgCcGCCCCAGACGa -3' miRNA: 3'- -AGUG-UCGAUaUCaa--GuUGGGGUUUGC- -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 12702 | 0.67 | 0.931009 |
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Target: 5'- -gACAGCUAUAuuggUCAAUUCCAGAUu -3' miRNA: 3'- agUGUCGAUAUca--AGUUGGGGUUUGc -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 2732 | 0.68 | 0.888349 |
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Target: 5'- gCACAGCaGUAGUUUGACCaaUAAACa -3' miRNA: 3'- aGUGUCGaUAUCAAGUUGGg-GUUUGc -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 21742 | 0.71 | 0.770993 |
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Target: 5'- gCGCGGUUAUcaauuGGUUCAACUCUggGCc -3' miRNA: 3'- aGUGUCGAUA-----UCAAGUUGGGGuuUGc -5' |
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| 6033 | 3' | -46.9 | NC_001813.1 | + | 876 | 1.1 | 0.003342 |
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Target: 5'- gUCACAGCUAUAGUUCAACCCCAAACGc -3' miRNA: 3'- -AGUGUCGAUAUCAAGUUGGGGUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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