Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6034 | 5' | -50.4 | NC_001813.1 | + | 14472 | 0.66 | 0.836788 |
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Target: 5'- uGCCGCaauugccGGAGCGACGAcuGGCgGUUCUa -3' miRNA: 3'- -CGGCG-------UCUUGUUGUUu-CUGaCGAGGa -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 1050 | 0.66 | 0.831965 |
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Target: 5'- uGCgGCGGcgucuucgcGCGGCAGGGGCUuugcuuuugcggccgGCUCCUg -3' miRNA: 3'- -CGgCGUCu--------UGUUGUUUCUGA---------------CGAGGA- -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 25168 | 0.66 | 0.82708 |
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Target: 5'- cGCCGCAGAAagguuuaugagauUGGCAGAaaacuGAgUGUUCCa -3' miRNA: 3'- -CGGCGUCUU-------------GUUGUUU-----CUgACGAGGa -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 21705 | 0.66 | 0.806954 |
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Target: 5'- gGCUGCAuuGGC-GCGAaaauucguauuuaAGACUGCUCCUu -3' miRNA: 3'- -CGGCGUc-UUGuUGUU-------------UCUGACGAGGA- -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 24028 | 0.67 | 0.754239 |
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Target: 5'- uCCGCAGAacaaaGCAGCAAAGGUUGCa--- -3' miRNA: 3'- cGGCGUCU-----UGUUGUUUCUGACGagga -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 11342 | 0.68 | 0.720128 |
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Target: 5'- aGCCacagaCGGAGCAACAGAaGgaGCUCCa -3' miRNA: 3'- -CGGc----GUCUUGUUGUUUcUgaCGAGGa -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 13567 | 0.72 | 0.48633 |
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Target: 5'- aGCUGCAGcGCAgccuGCAGcAGAUgagGCUCCUa -3' miRNA: 3'- -CGGCGUCuUGU----UGUU-UCUGa--CGAGGA- -5' |
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| 6034 | 5' | -50.4 | NC_001813.1 | + | 1089 | 1.1 | 0.001202 |
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Target: 5'- cGCCGCAGAACAACAAAGACUGCUCCUc -3' miRNA: 3'- -CGGCGUCUUGUUGUUUCUGACGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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