Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 604 | 3' | -45 | AC_000017.1 | + | 24341 | 0.66 | 0.993671 |
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Target: 5'- gGGACGAGagGCAUGGcgacuaccuAGAUgUgGGAGa -3' miRNA: 3'- -CCUGCUUa-CGUACCu--------UUUAgAaCCUC- -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 10476 | 0.66 | 0.991241 |
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Target: 5'- uGGugGAGgcGCGcGGAAAGUCgcGGAc -3' miRNA: 3'- -CCugCUUa-CGUaCCUUUUAGaaCCUc -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 21606 | 0.67 | 0.986281 |
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Target: 5'- uGGGCGGAguuUGCAUaGaGAAGGUUUUGGc- -3' miRNA: 3'- -CCUGCUU---ACGUA-C-CUUUUAGAACCuc -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 9761 | 0.67 | 0.984221 |
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Target: 5'- cGGGCGGcgGUcgGGGuuguuUCUggcGGAGg -3' miRNA: 3'- -CCUGCUuaCGuaCCUuuu--AGAa--CCUC- -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 4394 | 0.77 | 0.630914 |
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Target: 5'- gGGAUGggUGCAuacgUGGGGAuaugagaugcAUCUUGGAc -3' miRNA: 3'- -CCUGCuuACGU----ACCUUU----------UAGAACCUc -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 4548 | 0.82 | 0.38152 |
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Target: 5'- aGGA--AAUGCGUGGAAGAaCUUGGAGa -3' miRNA: 3'- -CCUgcUUACGUACCUUUUaGAACCUC- -5' |
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| 604 | 3' | -45 | AC_000017.1 | + | 4647 | 1.14 | 0.00366 |
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Target: 5'- uGGACGAAUGCAUGGAAAAUCUUGGAGg -3' miRNA: 3'- -CCUGCUUACGUACCUUUUAGAACCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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