Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6043 | 5' | -51.5 | NC_001813.1 | + | 28340 | 0.68 | 0.6077 |
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Target: 5'- --uGCUGCUGGCUGUc--GCugUGCUg -3' miRNA: 3'- gagUGGCGAUUGACGuuuCGugACGA- -5' |
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| 6043 | 5' | -51.5 | NC_001813.1 | + | 336 | 0.68 | 0.6077 |
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Target: 5'- uUCACCGCcAGCcucGCc--GCGCUGCUg -3' miRNA: 3'- gAGUGGCGaUUGa--CGuuuCGUGACGA- -5' |
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| 6043 | 5' | -51.5 | NC_001813.1 | + | 15494 | 0.71 | 0.426842 |
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Target: 5'- aUUACCG---GCaGCAGAGCACUGCa -3' miRNA: 3'- gAGUGGCgauUGaCGUUUCGUGACGa -5' |
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| 6043 | 5' | -51.5 | NC_001813.1 | + | 13627 | 0.72 | 0.40643 |
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Target: 5'- cCUCAuCUGCUGcagGCUGCGcuGCAgCUGCUg -3' miRNA: 3'- -GAGU-GGCGAU---UGACGUuuCGU-GACGA- -5' |
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| 6043 | 5' | -51.5 | NC_001813.1 | + | 22295 | 0.73 | 0.314078 |
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Target: 5'- -aUACCG-UGACUGCGAGGCACUcucGCUu -3' miRNA: 3'- gaGUGGCgAUUGACGUUUCGUGA---CGA- -5' |
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| 6043 | 5' | -51.5 | NC_001813.1 | + | 26803 | 1.09 | 0.000928 |
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Target: 5'- aCUCACCGCUAACUGCAAAGCACUGCUa -3' miRNA: 3'- -GAGUGGCGAUUGACGUUUCGUGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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